Direct enzyme-substrate affinity determination by real-time hyperpolarized (13)C-MRS.
نویسندگان
چکیده
A specialized kinetic analysis of real-time hyperpolarized [1,1,2,2-D4, 1-(13)C]choline (13)C-magnetic resonance spectroscopy enabled the determination of initial rates of metabolic enzyme activity (choline oxidase), enzyme-substrate affinity (Km), and inhibition. In a clinical MRI scanner, metabolite levels lower than 16 μM were detected at a temporal resolution of 1 s.
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عنوان ژورنال:
- Chemical communications
دوره 50 89 شماره
صفحات -
تاریخ انتشار 2014